G6795
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal
clone GSN24, purified from hybridoma cell culture
Sinonimo/i:
Anti-GFP
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About This Item
Codice UNSPSC:
12352203
NACRES:
NA.56
Prodotti consigliati
Origine biologica
mouse
Coniugato
unconjugated
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
GSN24, monoclonal
Stato
buffered aqueous solution
PM
antigen 27 kDa
Concentrazione
~2 mg/mL
tecniche
western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells
Isotipo
IgG1
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
2-8°C
modifica post-traduzionali bersaglio
unmodified
Descrizione generale
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.
Specificità
The antibody reacts specifically with GFP fusion proteins.
Applicazioni
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.
Azioni biochim/fisiol
Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stoccaggio e stabilità
For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
Esclusione di responsabilità
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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