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G6795

Sigma-Aldrich

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal

clone GSN24, purified from hybridoma cell culture

Sinonimo/i:

Anti-GFP

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.56

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

GSN24, monoclonal

Stato

buffered aqueous solution

PM

antigen 27 kDa

Concentrazione

~2 mg/mL

tecniche

western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells

Isotipo

IgG1

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C
2-8°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.

Specificità

The antibody reacts specifically with GFP fusion proteins.

Applicazioni

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.

Azioni biochim/fisiol

Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stoccaggio e stabilità

For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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