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G6784

Sigma-Aldrich

L-Glutamine–Penicillin–Streptomycin solution

L-glutamine 200 mM, streptomycin 10 mg/mL, penicillin 10,000 units, 0.1 μm filtered, BioReagent, suitable for cell culture

Sinonimo/i:

Pen/Strep, Penicillin/Streptomycin 

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About This Item

Numero MDL:
MFCD00283636
Codice UNSPSC:
12352207
NACRES:
NA.76

Origine biologica

synthetic

Livello qualitativo

300

Descrizione

stabilized

Sterilità

0.1 μm filtered

Nome Commerciale

BioReagent

Forma fisica

liquid

Composizione

L-glutamine, 200 mM
penicillin, 10,000 units
streptomycin, 10 mg/mL

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Spettro attività antibiotica

Gram-negative bacteria
Gram-positive bacteria

Modalità d’azione

cell wall synthesis | interferes
protein synthesis | interferes

Temperatura di conservazione

−20°C

Categorie correlate

Applicazioni

This antibiotic solution is used to reduce or eliminate bacterial contamination in primary cell cultures. It is recommended for us at 10 mL/L.

Azioni biochim/fisiol

Mode of Action: The product acts in three ways: Glutamine is an essential energy source for most mammalian cells in culture due to its amino acid carbon skeleton. Penicillin inhibits bacterial cell wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.

Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria.

Componenti

This solution is formulated to contain 200 mM L-glutamine, 10,000 units of Penicillin and 10 mg/mL of streptomycin, solubilized in a proprietary buffer.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Cells are covered with glycans. The expression and distribution of specific glycans on the surface of a cell are important for various cellular functions. Imaging these glycans is essential to aid elucidation of their biological roles. Here, utilizing methods of
N Massa et al.
Canadian journal of microbiology, 64(10), 647-663 (2018-05-11)
Candida glabrata is an opportunistic pathogen, associated with endocarditis, meningitis, and disseminated disease, and also with complicated vaginitis. Essential oils derived from aromatic plants are known in traditional medicine as antimicrobial agents and have antifungal properties. The aim of this
Nikoletta Sztán et al.
Reproduction, fertility, and development, 29(11), 2206-2216 (2017-03-28)
The conservation of genetic resources of avian species has become increasingly important over the past decade. The aim of the present study was to develop a genome preservation technique for the Hungarian goose Anser anser domestica. To this end, we
Chi-Fei Wang et al.
Anesthesia and analgesia, 107(4), 1397-1405 (2008-09-23)
Bulleyaconitine A (BLA) is an analgesic and antiinflammatory drug isolated from Aconitum plants. BLA has several potential targets, including voltage-gated Na+ channels. We tested whether BLA elicited long-lasting cutaneous analgesia, when co-injected with lidocaine and epinephrine, as a model for
Miriam Gonzalez Gonzalez et al.
Journal of immunological methods, 457, 73-77 (2018-02-25)
Standard cell culturing on plastic plates (two dimensional (2D) cultures) does not represent the actual microenvironment where cells reside in tissues. The three dimensional (3D) systems, composed of extracellular matrix and/or pure amino acids which form a scaffold for cells
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Articoli

L-Glutamine in Cell Culture

Importance and uses of glutamine in hybridoma and mammalian cell culture

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