G5166
PNGase F from Elizabethkingia miricola
buffered aqueous solution
Sinonimo/i:
N-Glycosidase F, Peptide N-glycosidase
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.32
Prodotti consigliati
Origine biologica
bacterial (Elizabethkingia miricola)
Livello qualitativo
Coniugato
(N-linked)
Stato
buffered aqueous solution
Attività specifica
≥20000 units/mg protein
PM
36 kDa
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.
Applicazioni
PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.
Used to deglycosylate protein.
Azioni biochim/fisiol
Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
Definizione di unità
One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.
Stato fisico
Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Articoli
N-Linked Glycan Strategies; Sigma-Aldrich.com
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