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Key Documents

F2253

Sigma-Aldrich

Riboflavin 5′-monophosphate sodium salt hydrate

synthetic, ≥70% (HPLC)

Sinonimo/i:

FMN-Na, Flavin mononucleotide, Riboflavin 5′-phosphate sodium salt

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About This Item

Formula empirica (notazione di Hill):
C17H20N4NaO9P · xH2O
Numero CAS:
Peso molecolare:
478.33 (anhydrous basis)
Beilstein:
4106529
Numero MDL:
Codice UNSPSC:
41106305
eCl@ss:
34058005
ID PubChem:
NACRES:
NA.51

Origine biologica

synthetic

Livello qualitativo

Saggio

≥70% (HPLC)

Forma fisica

powder

Impurezze

≤0.3% Riboflavin

Solubilità

water: 50 mg/mL

Temperatura di conservazione

−20°C

Stringa SMILE

O.[Na+].Cc1cc2N=C3C(=O)NC(=O)N=C3N(C[C@H](O)[C@H](O)[C@H](O)COP(O)([O-])=O)c2cc1C

InChI

1S/C17H21N4O9P.Na.H2O/c1-7-3-9-10(4-8(7)2)21(15-13(18-9)16(25)20-17(26)19-15)5-11(22)14(24)12(23)6-30-31(27,28)29;;/h3-4,11-12,14,22-24H,5-6H2,1-2H3,(H,20,25,26)(H2,27,28,29);;1H2/q;+1;/p-1/t11-,12+,14-;;/m0../s1
BHRVCJBIICJWTH-APQIITSESA-M

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Applicazioni

Riboflavin 5′-monophosphate sodium salt hydrate has been used:
  • as a component of assay buffer to determine the luminescence of L. lactis cells
  • as a component of the reaction mixture in nitric oxide synthase (NOS) enzymatic activity assay
  • in the high performance liquid chromatography (HPLC) analysis of Flavin mononucleotide (FMN) cyclase products
  • in luciferase assay with firefly luciferase

Azioni biochim/fisiol

Riboflavin 5′-monophosphate (FMN), a derivative of riboflavin, is used as a prosthetic group for one and two-electron transfer in oxidation/reduction reactions. It acts as a cofactor for enzymes such as reduced nicotinamide adenine dinucleotide (NADH) dehydrogenase, nitric oxide synthase, and nitrilotriacetate monooxygenase. Riboflavin 5′-monophosphate is used as a substrate to study the specificity and kinetics of FMN phosphohydrolases and to study molecular sensing-based gene regulation by riboswitches.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


Certificati d'analisi (COA)

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Luciferase detection during stationary phase in Lactococcus lactis
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Establishment of patterned thalamocortical connections does not require nitric oxide synthase
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