E2886
ExtrAvidin®−Peroxidase
buffered aqueous solution
Sinonimo/i:
ExtrAvidin HRP
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About This Item
Prodotti consigliati
Coniugato
peroxidase conjugate
Livello qualitativo
Forma fisica
buffered aqueous solution
tecniche
direct ELISA: 1:2,000
dot blot: 1:4,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
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Specificità
The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.
Applicazioni
ExtrAvidin®-Peroxidase is suitable for the following applications:
- Dot blot at a minimum dilution of 1:4,000
- Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
- Direct ELISA at a minimum dilution of 1:2,000
- Immunoprecipitation and Immunobloting
- Ra2I solid-phase binding assays
- Southwestern blotting
- Western blotting
- Immunocytochemistry
Azioni biochim/fisiol
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Nota sulla preparazione
Affinity purified protein
Stoccaggio e stabilità
Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Note legali
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Protocolli
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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