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D2906

Sigma-Aldrich

DMEM/F-12

With ʟ-glutamine, sodium pyruvate and HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Sinonimo/i:

DME/F-12, miscela 1:1

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About This Item

Numero MDL:
MFCD00217417
Codice UNSPSC:
12352207
NACRES:
NA.75

Nome del prodotto

Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Livello qualitativo

500

Stato

powder

tecniche

cell culture | mammalian: suitable

Componenti

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Descrizione generale

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

Applicazioni

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

  • Hep-2 cells
  • mouse fibroblast L‐cells
  • L‐ Wnt family member 3A (Wnt3a) cells
  • primary buffalo embryonic fibroblast (BEF)

Quantità

Formulated to contain 15.6 grams of powder per liter of medium.

Ricostituzione

Supplement with 1.2 g/L sodium bicarbonate.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

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Certificati d'analisi (COA)

Lot/Batch Number
SLCL9192
SLCL9194
SLCL8144
SLCC3739
SLBW3270

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Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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