M15

95102517, mouse kidney, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: M15, 95102517
• Origine biologica: mouse kidney
• Modalità di accrescimento: Adherent
• Cariotipo: Hyperdiploid
• Morfologia: Epithelial
• Prodotti: WT1 (expressed gene)
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse mesonephric epithelium, polyoma virus large T transformed

Descrizione della linea cellulare

The WT1-expressing mesonephric cell line M15 (alias Meso15) was established from mouse mesonephros transgenically expressing the large T protein of polyoma virus under the control of the early viral enhancer. As a tumour suppresser gene with a key role in urogenital development, WT1 is implicated as predisposition gene in the pathogenesis of Wilms′ tumour (WT).

Applicazioni

Gene expression and protein studies connected to kidney development and Wilms′ tumourigenesis.

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. NB: Cell growth is impaired at lower densities.

Altre note

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