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Key Documents

A6582

Sigma-Aldrich

Antifoam A concentrate

active silicone polymer 100%

Sinonimo/i:

Antifoam agent, Antifoam solution, Foam control agent

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About This Item

Codice UNSPSC:
12161901
NACRES:
NA.85

Livello qualitativo

Forma fisica

emulsion (aqueous)

tecniche

cell culture | hybridoma: suitable
microbiological culture: suitable

Densità

0.97 g/mL at 25 °C

applicazioni

microbiology

Temperatura di conservazione

room temp

Compatibilità

(Mammalian (suspension))
bacteria (fermentation)

Descrizione generale

Antifoam A is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production. Extremely effective for aqueous and non-aqueous systems. Effective at concentrations in the 1-50ppm range.

Applicazioni

Antifoam A concentrate has been used:
  • in bioreactor culture for Staphylococcal enterotoxin C (SEC) production.
  • to prevent foam formation during the aerobic treatment of sludge.
  • to study the impact of surface tension on bioaerosol generation during bubbling.

Caratteristiche e vantaggi

  • Molecular biology grade and tested for use in bacterial fermentation.
  • Extremely effective foam suppressor for aqueous and non-aqueous systems.
  • Made of 100% active silicone polymer, free of emulsifiers.
  • Typically, effective at 1-100 ppm.
  • Product will be stable in the pH range of 5 to 9.
  • Can be directly added to a fermentation medium but it is not recommended that it be pumped to a fermenter on an as-needed basis.

Componenti

Antifoam A is a 100% active silicon polymer containing no non-ionic emulsifiers .

Nota sulla preparazione

Antifoam A can be pre-diluted with 3-10 parts of aqueous propylene glycol or non-aqueous vegetable oil with slow mixing.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Environment

Avvertenze

Warning

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Aquatic Acute 1 - Aquatic Chronic 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

>213.8 °F - closed cup

Punto d’infiammabilità (°C)

> 101 °C - closed cup

Dispositivi di protezione individuale

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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M Schlömann et al.
Journal of bacteriology, 172(9), 5119-5129 (1990-09-01)
Enzymatic conversion of 4-fluorocatechol in the simultaneous presence of partially purified preparations of catechol 1,2-dioxygenase from Pseudomonas cepacia and muconate cycloisomerase from Alcaligenes eutrophus 335 yielded a product that was unambiguously identified as (+)-4-fluoromuconolactone [(+)-4-carboxymethyl-4-fluoro-but-2-en-4-olide]. This compound was shown to
F P Ernani et al.
Infection and immunity, 61(6), 2596-2601 (1993-06-01)
Substantial evidence indicates that molecules released by infectious organisms affect virulence and influence immunity to infection. The characterization of extracellular molecules and their mechanism of release is therefore critical to understanding host-parasite relationships. The cestode parasite Mesocestoides corti is known
Experience with a perfo-suction system for the recovery of schistosomes from laboratory rodents
T. F. H. G. Jackson, C. D. Dettman, et al.
Laboratory Animal Science, 16, 65-67 (1982)
Factors affecting staphylococcal enterotoxin C bovine production in milk
Hunt K, et al.
International dairy journal, 39(1), 41-46 (2014)
Sarah J Routledge et al.
Microbial cell factories, 10, 17-17 (2011-03-24)
Pichia pastoris is a widely-used host for recombinant protein production. Initial screening for both suitable clones and optimum culture conditions is typically carried out in multi-well plates. This is followed by up-scaling either to shake-flasks or continuously stirred tank bioreactors.

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