C2-Rev 7

94101907

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: rat liver
• Modalità di accrescimento: Adherent
• Cariotipo: Hyperdiploid, 51 chromosomes
• Morfologia: Epithelial
• Prodotti: Albumin and hepatocyte markers
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat hepatoma [HGPRT-]

Descrizione della linea cellulare

C2-Rev 7 is a revertant of the C2 variants, selected by virtue of its capacity to grow in glucose free medium (containing dialysed serum) where survival requires activity of the liver-specific gluconeogenic pathway. Expression of the entire set of liver specific functions is restored. Albumin and hepatocyte markers present.

Applicazioni

Gene expression studies.

Terreno di coltura

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split semi-confluent cultures (70%) 1:10 i.e. seeding at 9x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1,000,000 cells/10cm² dish.

Altre note

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