C2

94101906, rat liver, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: C2, 94101906
• Origine biologica: rat liver
• Modalità di accrescimento: Adherent
• Cariotipo: Hyperdiploid, 52 Chromosomes
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat hepatoma [HGPRT-]

Descrizione della linea cellulare

Full name Faof1C2 is a dedifferentiated variant of Fao. These cells continue to express tyrosine aminotransferase, but the liver-specific markers are mostly absent.

Applicazioni

Gene expression analysis.

Terreno di coltura

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split semi-confluent cultures 1:50 i.e. seeding at 2x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 10x10,000,000,000 cells/10cm² dish.

Altre note

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