DK

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: dog kidney
• Confezionamento: tube of 5 μg 93120836-DNA-5UG; pkg of vial of cells 93120836-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Canine kidney

Descrizione della linea cellulare

Derived from the kidney cortex of a 6 week old Beagle of unknown sex. The cells support the growth of many viruses including influenza, parainfluenza-2, infectious bovine rhinotracheitis, canine hepatitis, adenovirus, vaccinia and VSV (Indiana strain).

Applicazioni

DK cell line has been used to study the functional organization of the major late transcriptional unit of canine adenovirus type 2 (CAV-2).

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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