RO82-W-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human thyroid
• Confezionamento: tube of 5 μg 92030502-DNA-5UG; pkg of vial of cells 92030502-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 60-82, 82 predominant
• Morfologia: Epithelial
• Prodotti: Thyroglobulin
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: metastasis
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human follicular thryoid carcinoma

Descrizione della linea cellulare

Established from the metastases of a follicular carcinoma in a female patient. Tumorigenic in nude mice. The basal release of immuno-detectable thyroglobulin increased in response to thyroid-stimulating hormone (TSH). The cell line grows as a monolayer without the formation of follicles. Electronmicroscopy studies showed the presence of microvilli with interdigitations between neighbouring cells.

Applicazioni

A valuable model for the study of human follicular carcinoma.

Terreno di coltura

DMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.