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Documenti fondamentali

U-2 OS Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92022711, human bone, Not specified

Sinonimo/i:

U-2OS Cells, U2-OS Cells, U20S Cells, U2OS Cells

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About This Item

Codice UNSPSC:
41106514

Origine biologica

human bone

Confezionamento

tube of 5 μg 92022711-DNA-5UG
pkg of vial of cells 92022711-1VL

Modalità di accrescimento

Adherent

Cariotipo

Not specified

Morfologia

Not specified

Prodotti

Not specified

Recettori

Not specified

tecniche

cell culture | mammalian: suitable

Malattie correlate

cancer

Condizioni di spedizione

dry ice

Temperatura di conservazione

−196°C

Categorie correlate

Origine della linea cellulare

Human Osteosarcoma

Descrizione della linea cellulare

Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

Applicazioni

U-2 OS has been used to study:
  • the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
  • the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
  • calcium-mediated actin reset (CaAR) in response to physiological changes

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18

Terreno di coltura

McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.

Altre note

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Articoli

Cell Cycle Analysis Using a Nucleoside Triphosphate (NTP) Transporter Molecule for Rapid DNA Labeling in Living Cells

Regulation of the cell cycle involves processes crucial to the survival of a cell, including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division associated with cancer. The cell cycle is a four-stage process in which the cell 1) increases in size (G1-stage), 2) copies its DNA (synthesis, S-stage), 3) prepares to divide (G2-stage), and 4) divides (mitosis, M-stage). Due to their anionic nature, nucleoside triphosphates (NTPs), the building blocks of both RNA and DNA, do not permeate cell membranes.

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