BB

87101201, fish trunk, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: BB, 87101201
• Origine biologica: fish trunk
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 60
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Fish Brown Bullhead posterior trunk tissue

Descrizione della linea cellulare

Derived from the trunk tissue (excluding fins) of a 2 year old Brown Bullhead fish. These cells will tolerate a temperature range of 4-34°C. The optimal temperature range for cell proliferation is 25-30°C. BB is susceptible to infectious pancreatic necrosis virus and channel catfish virus.

Applicazioni

Virus studies: Infectious pancreatic necrotis virus and Channel catfish virus and channel catfish virus

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO₂; 23°C. Note: The cells do not grow well on glass. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for a

Altre note

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