EPC

93120820, fish skin, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: EPC, from fish skin, 93120820
• Origine biologica: fish skin
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 96
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice

Descrizione

Origine della linea cellulare

Fish epithelioma Fathead minnow Pimephales promelas

Descrizione della linea cellulare

This cell line known as Epithelioma papulosum cyprini (EPC) was originally reported to be from Carp (Cyprinus carpio) epidermal herpes virus-induced hyperplastic lesions. More recently EPC was found to be derived from Fathead Minnow (Pimephales promelas) see Winton et al., 2010. This has been confirmed by DNA sequencing at ECACC. The cells support the growth of pancreatic necrosis virus and rhabdovirus.

Profilo DNA

Not specified

Terreno di coltura

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO₂; 20 °C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

Altre note

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