MiCL1 (S+L-)

86020603, mink lung, Fibroblast-like

• Codice UNSPSC: 41106514

Proprietà

• product name: MiCL1 (S+L-), 86020603
• Origine biologica: mink lung
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 30
• Morfologia: Fibroblast-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mink lung, Moloney murine sarcoma virus infected

Descrizione della linea cellulare

A clone of the mink lung cell line Mv.1.Lu (Sigma Catalogue number. 88050503). Isolated from a single cell of a focus produced in the monolayer after infection with Moloney murine sarcoma virus associated with the RD-114 helper leukaemia virus. Super infection with non-transforming leukaemia virus results in transformation and net amplification of sarcoma-specific RNA sequences.

Applicazioni

Virus studies: Moloney MSV and RD-114 helper leukaemia virus Leukemogenesis

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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