B82

85011408, mouse adipose tissue, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: B82, 85011408
• Origine biologica: mouse adipose tissue
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 51
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse C3H/An sc areolar and adipose tissue

Descrizione della linea cellulare

Derivative of NCTC clone 929 (Sigma Catalogue number. 88102702) obtained by progressive selection in BUdR, has a cloning efficiency of 70-80%.

Applicazioni

Chromosome analysis and hybridisation studies

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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