PNT2 (SERUM FREE)

7042701, human prostate, Epithelial

• Codice UNSPSC: 41106514
• Origine biologica: human prostate
• Modalità di accrescimento: Adherent
• Cariotipo: Not
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified

Proprietà

• Nome del prodotto: PNT2 (SERUM FREE), 07042701
• Origine biologica: human prostate
• Modalità di accrescimento: Adherent
• Cariotipo: Not
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human prostate normal, serum-free

Descrizione della linea cellulare

This cell line is a derivative of the normal human prostate cell line PNT2 (Sigma cat no. 95012613) adapted to grow in serum-free medium. PNT2 was established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein.

Terreno di coltura

Defined Keratinocyte-SFM (Gibco 10785) + Growth Supplement (Gibco 10784-015).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Use Trypsin Inhibitor to inactivate the trypsin. When freezing cells down use conditioned medium plus 10% DMSO.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.