C2888
Acetylcholinesterase from Electrophorus electricus (electric eel)
Type V-S, lyophilized powder, ≥1,000 units/mg protein
Sinonimo/i:
AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
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About This Item
Prodotti consigliati
Origine biologica
Electrophorus electricus
Livello qualitativo
Tipo
Type V-S
Forma fisica
lyophilized powder
Attività specifica
≥1,000 units/mg protein
PM
280 kDa
Composizione
Protein, ≥45% biuret
pI
~5.3
Solubilità
Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
Temperatura di conservazione
−20°C
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Descrizione generale
Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Applicazioni
Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
- in acetylcholinesterase activity assay
- to study its effects on Xenopus embryonic hippocampal cells
- to study the effect of acetylcholine inhibitors on development and plasticity
Azioni biochim/fisiol
Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
Avvertenza
For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.
Definizione di unità
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
Stato fisico
Lyophilized powder containing Tris buffer salts
Risultati analitici
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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