C2780
Chondroitinase AC from Flavobacterium heparinum
lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
Sinonimo/i:
Chondroitin AC lyase
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Coniugato
(Glucosaminoglycan)
Livello qualitativo
Stato
lyophilized powder
Attività specifica
0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
Composizione
Protein, ~15% Lowry
Solubilità
0.02 M phosphate buffer: soluble (pH 7.0)
Attività estranea
Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount
Temperatura di conservazione
−20°C
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Applicazioni
Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.
Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.
Azioni biochim/fisiol
Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
Altre note
Contains potassium phosphate buffer salts and BSA as stabilizer.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Compatibilità
Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides
Definizione di unità
One unit will cause a ΔA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).
Nota sulla preparazione
Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.
Applicazioni
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Articoli
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
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