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Key Documents

C2272

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

biotin conjugate, Type IV, lyophilized powder

Sinonimo/i:

ConA

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

Origine biologica

Canavalia ensiformis

Livello qualitativo

Coniugato

biotin conjugate

Tipo

Type IV

Forma fisica

lyophilized powder

Composizione

Protein, ~85% ε1%/280

Grado di funzionalizzazione

4-8 mol biotin per mol protein

Impurezze

Carbohydrate, essentially free

Temperatura di conservazione

2-8°C

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Descrizione generale

Concanavalin A is isolated from the from Canavalia ensiformis (Jack bean). It is a mitogenic lectin with a dome-like structures and is a tetrameric protein.

Applicazioni

Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
  • in the colabelling of fischer rat thyroid (FRT) epithelia(104)
  • to induce autoimmune hepatitis in Cre-conditional atg7 knockout mice(105)
  • to detect glycoproteins containing α-D-mannose, α-D-glucose when used with avidin or streptavidin detection system(106)

Azioni biochim/fisiol

Concanavalin A (Con A) bound to glycoenzyme has wide applications and can be dissociated in the presence of high sugar levels. Con A induces hepatic inflammation. It stimulates macrophages and promotes cytokine production. Con A also exhibits anti-hepatoma effect and could be used effectively as a vaccine for treating cancer.
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Confezionamento

Package size based on protein content

Stato fisico

Contains sodium citrate

Risultati analitici

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Certificati d'analisi (COA)

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Hillary Claire Tunggal et al.
PloS one, 16(6), e0253265-e0253265 (2021-06-18)
A therapeutic vaccine that induces lasting control of HIV infection could eliminate the need for lifelong adherence to antiretroviral therapy. This study investigated a therapeutic DNA vaccine delivered with a single adjuvant or a novel combination of adjuvants to augment
Concanavalin A: a useful ligand for glycoenzyme immobilization?a review
Saleemuddin, M and Husain Q
Enzyme and Microbial Technology, 13(4), 290-295 (1991)
Jens Friedrichs et al.
Nature protocols, 5(7), 1353-1361 (2010-07-03)
Atomic force microscopy (AFM)-based single-cell force spectroscopy (SCFS) enables the quantitative study of cell adhesion under physiological conditions. SCFS probes adhesive interactions of single living cells with substrates such as extracellular matrix (ECM) proteins and other cells. Here we present
Glycoproteins from the cuticle of the Atlantic shore crab Carcinus maenas: I. Electrophoresis and Western-blot analysis by use of lectins
Compere P, et al.
The Biological Bulletin, 202(1), 61-73 (2002)
Atg7 knockdown augments concanavalin A-induced acute hepatitis through an ROS-mediated p38/MAPK pathway
Zhuang Y, et al.
PLoS ONE, 11(3), e0149754-e0149754 (2016)

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