C0581
Monoclonal Anti-Calcineurin (β-Subunit) antibody produced in mouse
clone CN-B1, ascites fluid
Sinonimo/i:
Anti-CALNB1, Anti-CNB, Anti-CNB1
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About This Item
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
unconjugated
Forma dell’anticorpo
ascites fluid
Tipo di anticorpo
primary antibodies
Clone
CN-B1, monoclonal
contiene
15 mM sodium azide
Reattività contro le specie
bovine, rat, human, mouse
tecniche
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using neurons in human myenteric and prostatic ganglia
indirect ELISA: suitable
western blot: 1:3,000 using rat brain extract
Isotipo
IgG2b
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... PPP3R1(5534) , PPP3R2(5535)
rat ... Ppp3r1(29748) , Ppp3r2(29749)
Descrizione generale
Calcineurin (CaN, heterodimeric enzyme) is a Ca(2+)/calmodulin (CaM)-stimulated protein phosphatase 3 (PPP3) consisting of a catalytic A subunit (CnA) and a Ca(2+)-binding regulatory B subunit (CnB). The calcium-binding regulatory subunit (calcineurin B) is coded by the PPP3R1 gene located on human chromosome 2. Protein phosphatase 3, regulatory subunit B, α (PPP3R1) is also known as CALN, CCN1, CNA1, CALNA, PPP2B and CALNA1. The regulatory subunit (calcineurin B) is widely expressed in brain while the catalytic subunit (calcineurin A) is abundantly expressed in Hassall′s corpuscles, localized in the thymic medulla and represents the terminal stages of thymic medullary epithelium.
Monoclonal Anti-Calcineurin (β-subunit) (mouse IgM isotype) is derived from the CN-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified bovine brain calcineurin preparation. Two different calcineurin B has been reported in rat testis.
Specificità
In immunoblotting, the antibody recognizes an epitope located on the β-subunit of calcineurin (18 kDa, also called calcineurin B) and does not cross-react with the α-subunit.
Immunogeno
bovine brain calcineurin
Applicazioni
Monoclonal Anti-Calcineurin (b-Subunit) antibody produced in mouse has been used in immunohistochemistry, indirect ELISA and western blotting.
Azioni biochim/fisiol
Calcineurin calcium -binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. immunoreactivity is detected at significant concentrations only in normal and neoplastic neurons regions of the cytoplasm dendrites, axons and axon terminals. A close correlation has been observed between inhibition of calcineurin by the complexes of cyclosporin A/cyclophilin and tacrolimus(FK506) and FK506 binding proteins (FKBPs) and inhibition of apoptosis. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage, such as an ischemic insult.
Calcineurin is a major soluble calmodulin-binding protein in the brain. It stimulates the expression of the surface molecules CD83, CD80, CD86, CD40 and HLA-DR by promoting secretion of inflammatory cytokines IL-6, TNF-α and IL-1β by human PBMC-derived dendritic cells. It plays an important role in signal transduction, activation of T cell. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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The unique C terminus of the calcineurin isoform CNAbeta1 confers non-canonical regulation of enzyme activity by Ca2+ and calmodulin
Bond R, et al.
The Journal of biological chemistry, 292(40), 16709-16721 (2017)
Rachel Bond et al.
The Journal of biological chemistry, 292(40), 16709-16721 (2017-08-27)
Calcineurin, the conserved Ca2+/calmodulin-regulated phosphatase and target of immunosuppressants, plays important roles in the circulatory, nervous, and immune systems. Calcineurin activity strictly depends on Ca2+ and Ca2+-bound calmodulin (Ca2+/CaM) to relieve autoinhibition of the catalytic subunit (CNA) by its C
Substitution in position 3 of cyclosporin A abolishes the cyclophilin-mediated gain-of-function mechanism but not immunosuppression
Baumgrass R, et al.
The Journal of Biological Chemistry, 279(4), 2470-2479 (2004)
Neural functions of calcineurin in synaptic plasticity and memory
Baumgartel K and Mansuy IM
Learning & Memory, 19(9), 375-384 (2012)
Protein phosphatase 2B (PP2B, calcineurin) in Paramecium: partial characterization reveals that two members of the unusually large catalytic subunit family have distinct roles in calcium-dependent processes
Fraga D, et al.
Eukaryotic Cell, 9(7), 1049-1063 (2010)
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