B7263

N-tert-Butyl-α-phenylnitrone

≥98% (GC)

• Sinonimo/i: N-Benzylidene-tert-butylamine N-oxide, PBN, Phenyl N-t-butylnitrone
• Formula condensata: C₆H₅CH=N(O)C(CH₃)₃
• Numero CAS: 3376-24-7
• Peso molecolare: 177.24
• Beilstein: 2044028
• Numero CE: 222-168-6
• Numero MDL: MFCD00008799
• Codice UNSPSC: 12352202
• ID PubChem: 24891981
• NACRES: NA.77

Proprietà

• Saggio: ≥98% (GC)
• Forma fisica: powder
• Colore: white
• Punto di fusione: 73-74 °C (lit.)
• Solubilità: DMSO: soluble
• Temperatura di conservazione: −20°C
• Stringa SMILE: CC(C)(C)[N+](\[O-])=C\c1ccccc1
• InChI: 1S/C11H15NO/c1-11(2,3)12(13)9-10-7-5-4-6-8-10/h4-9H,1-3H3/b12-9-; IYSYLWYGCWTJSG-XFXZXTDPSA-N

Descrizione

Applicazioni

N-tert-Butyl-α-phenylnitrone has been used:

• as a component of Dneasy Blood&Tissue buffer to preserve the oxidized state of DNA extracted from human non-tumorigenic epithelial breast (MCF10A) cells
• as a free radical scavenger of reactive oxygen species (ROS) in microglial (MG) cell lines
• to attenuate fibroblast senescence in unstable oral squamous cell carcinomas (GU-OSCC)

Azioni biochim/fisiol

N-tert-butyl-α-phenylnitrone (PBN) is a commonly used free-radical spin trap. It has been shown to reduce the number of emboli-induced cerebral microinfarctions in the rabbit cortex and prevent neoplasia by its radical scavenging activity and its ability to inhibit cyclooxygenase-2 activity. Reported to inhibit the induction of nitric oxide synthase (iNOS), thereby preventing the overproduction of nitric oxide (NO). PBN in a dose of 100 mg/kg i.p. reduced necrosis of the substantia nigra, pars reticulate in flurothyl-induced status epilepticus in rats. It protects against some types of post-trauma epileptogenic events in an animal model of epilepsy. The lethal dose of PBN in rats was found to be approximately 100 mg/100 g body weight (0.564 mmol/100Å g).

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)