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Documenti fondamentali

B6901

Sigma-Aldrich

Monoclonal Anti-Bovine IgG antibody produced in mouse

clone BG-18, ascites fluid

Sinonimo/i:

Monoclonal Anti-Bovine IgG

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

secondary antibodies

Clone

BG-18, monoclonal

contiene

15 mM sodium azide

Reattività contro le specie

bovine (non-reactive to IgM)

Non deve reagire con

pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat

tecniche

dot blot: suitable
indirect ELISA: 1:1000

Isotipo

IgG1

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Descrizione generale

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.
The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction. IgG (immunoglobulin G) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical γ or γ light (L) chains of 50 kDa and 25 kDa, respectively.

Specificità

bovine IgG1 and IgG2

Immunogeno

Purified bovine IgG

Applicazioni

Monoclonal Anti-Bovine IgG antibody has been used in indirect immunoperoxidase staining.
Monoclonal Anti-Bovine IgG antibody produced in mouse is suitable for ELISA at a working dilution of 1:20,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. Monoclonal Anti-Bovine IgG antibody produced in mouse was used as an isotype control in ChIP assay.

Azioni biochim/fisiol

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Stato fisico

Ascites fluid containing 0.1% sodium azide

Stoccaggio e stabilità

For continuous use, store at 2-8°C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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