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B1645

Sigma-Aldrich

Anti-Bovine IgG (whole molecule) antibody produced in goat

whole antiserum

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

goat

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

whole antiserum

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

contiene

15 mM sodium azide

tecniche

quantitative precipitin assay: 2.8 mg/mL

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.

Specificità

The antiserum shows a single arc of precipitation versus bovine serum and bovine IgG by immunoelectrophoresis (IEP). This antiserum has not been assayed for interspecies cross reactivity.

Applicazioni

Anti-Bovine IgG (whole molecule) antibody produced in goat has been used in:
  • quantitative precipitation assays and in various immunosensor methods to detect bovine IgG in serum and milk
  • immuno-reaction assay
  • indirect sandwich enzyme linked immunosorbent assay (ELISA)
It has also been used in immuno-reaction assay.

Azioni biochim/fisiol

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Stato fisico

Supplied as a liquid with 0.1% sodium azide (see MSDS) as a preservative.

Nota sulla preparazione

treated to remove lipoproteins

Stoccaggio e stabilità

For continuous use, store at 0-5 °C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Mauro Tomassetti et al.
Journal of pharmaceutical and biomedical analysis, 73, 90-98 (2012-05-09)
Within the framework of research carried out by our team aimed at developing new immunological methods to determine proteins such as immunoglobulins G in different biological matrixes, for instance, serum and milk, tests were performed on several immunosensors based on
Bismuth film electrode as sensing platform for IgE-anti-IgE interactions
Anik U, et al.
Electroanalysis, 23(10), 2379-2385 (2011)
IL-21-induced isotype switching to IgG and IgA by human naive B cells is differentially regulated by IL-4
Avery DT, et al.
Journal of Immunology, 181(3), 1767-1779 (2008)
Placental transfer of IgG subclasses in a Japanese population.
Hashira S, et al.
Pediatrics International, 42(4) (2000)
Gestur Vidarsson et al.
Frontiers in immunology, 5, 520-520 (2014-11-05)
Of the five immunoglobulin isotypes, immunoglobulin G (IgG) is most abundant in human serum. The four subclasses, IgG1, IgG2, IgG3, and IgG4, which are highly conserved, differ in their constant region, particularly in their hinges and upper CH2 domains. These

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