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Key Documents

B6061

Sigma-Aldrich

Anti-Beclin 1 (N-terminal)

IgG fraction of antiserum, buffered aqueous solution

Sinonimo/i:

Anti-ATG6, Anti-BECN1, Anti-Coiled-coil myosin-like BCL2-interacting protein, Anti-GT197, Anti-VPS30

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Coniugato

unconjugated

Forma dell’anticorpo

IgG fraction of antiserum

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

PM

antigen ~60 kDa

Reattività contro le specie

human

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtrAvidin®-Peroxidase staining of heat-retrieved sections of human cerebellum
immunoprecipitation (IP): 1-2 μL using extract of human HEK-293T cells expressing recombinant human Beclin 1 fusion protein
indirect immunofluorescence: suitable
western blot: 1:250-1:500 using whole extract of human HeLa cells

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... BECN1(8678)

Descrizione generale

Beclin 1 (BECN1) or GT197 is a tumor suppressor gene. It is a Bcl-2 (B-cell lymphoma 2) interacting protein and is the mammalian ortholog of yeast Atg6/Vps30. It is a 450 amino-acid protein having three domains. The gene encoding BECN1 is localized on human chromosome 17q21.

Immunogeno

synthetic peptide corresponding to amino acids 29-46 of human Beclin 1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Applicazioni

Anti-Beclin 1 (N-terminal) antibody produced in rabbit has been used in:
  • immunofluorescent staining
  • immunoblotting
  • immunohistochemistry
  • immunoprecipitation

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Azioni biochim/fisiol

Beclin 1 (BECN1) is able to complement the autophagy deficiency of yeast lacking autophagy-related gene 6 (Atg6) and restores autophagy in human MCF7 breast carcinoma cells. Beclin 1 plays a major role in the early development of embryo. It also serves as a haplo-insufficient tumor-suppressor gene. Heterozygous Beclin 1 mutant mice have increased spontaneous tumorigenesis. Beclin 1 is monoallelically deleted in a high percentage of sporadic human breast, ovarian and prostate carcinomas and is expressed at reduced levels in those tumors. In human brains BECN1 decreases in an age-dependent fashion, possibly leading to a reduction of autophagic activity during aging, which may contribute to the accumulation of mutant Huntingtin and the age-delayed disease onset of Huntington disease.

Stato fisico

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Note legali

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificati d'analisi (COA)

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Sigma-Aldrich

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bis-Dehydroxy-Curcumin triggers mitochondrial-associated cell death in human colon cancer cells through ER-stress induced autophagy
Basile V, et al.
Testing, 8(1), e53664-e53664 (2013)
Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor
Yue Z, et al.
Proceedings of the National Academy of Sciences of the USA, 100(25), 15077-15082 (2003)
Jinghua Wu et al.
Oncology letters, 13(2), 770-776 (2017-03-31)
The interleukin (IL)-17/IL-17 receptor (IL-17R) complex has been shown to be important for the regulation of inflammation; however, its role in the regulation of tumor processes has recently emerged as a research focus. The present study demonstrated that oxaliplatin was
The BECN1 N-terminal domain is intrinsically disordered
Lee EF, et al.
Autophagy, 3-3 (2016)
Proper autophagy is indispensable for angiogenesis during chick embryo development
Lu W, et al.
Cell Cycle, 15(13), 1742-1754 (2016)

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