Passa al contenuto
Merck
Tutte le immagini(2)

Key Documents

View All Documentation

A7646

Sigma-Aldrich

Apirasi

ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

Sinonimo/i:

Adenosina 5′-difosfatasi, Adenosina 5′-trifosfatasi

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(2)

About This Item

Numero CAS:
9000-95-7
Classificazione EC (Enzyme Commission):
3.6.1.5 (BRENDA, IUBMB)
Numero CE:
232-569-8
Numero MDL:
MFCD00130542
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

potato

Livello qualitativo

200

Tipo

Grade III

Forma fisica

lyophilized powder (partially purified)

ATPase activity

≥60 units/mg protein

PM

~49 kDa by gel filtration

Composizione

Protein, 30-60%

Solubilità

H2O: soluble 10 mg/mL, slightly hazy

applicazioni

diagnostic assay manufacturing

Attività estranea

Acid Phosphatase ≤5% of base activity

Temperatura di conservazione

−20°C

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Applicazioni

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.
In diverse varietà di S. tuberosum si trovano almeno due isoenzimi: uno con un rapporto ATPasi/ADPasi elevato (∼10) e un altro con un rapporto basso (∼1).
Reazione: ATP → ADP+Pi → AMP+2Pi.

Azioni biochim/fisiol

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.

Confezionamento

Sold on the basis of ATPase units

Altre note

Mixture of high and low ratio isoenzymes.

Qualità

Contains ≤ 5% acid phosphatase.

Definizione di unità

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Yen-Ta Chen et al.
Journal of tissue engineering and regenerative medicine, 13(12), 2155-2168 (2019-09-11)
This study tested the hypothesis that preactivated and disaggregated shape-changed platelet (PreD-SCP) therapy significantly protected rat kidney from ischemia-reperfusion (IR) injury. Adult-male Sprague-Dawley rats (n = 24) were equally categorized into Groups 1 (sham-operated control [SC]), 2 (SC + PreD-SCP)
Properties of Two Apyrases from Solanum tuberosum
Kettlun, A. et al.
Phytochemistry, 21(3), 551-558 (1982)
Koji Atarashi et al.
Nature, 455(7214), 808-812 (2008-08-22)
Interleukin (IL)-17-producing CD4(+) T lymphocytes (T(H)17 cells) constitute a subset of T-helper cells involved in host defence and several immune disorders. An intriguing feature of T(H)17 cells is their selective and constitutive presence in the intestinal lamina propria. Here we
Mathieu F Chevalier et al.
Blood, 121(1), 29-37 (2012-10-09)
Natural regulatory T cells (Tregs) participate in responses to various chronic infections including HIV. HIV infection is associated with a progressive CD4 lymphopenia and defective HIV-specific CD8 responses known to play a key role in the control of viral replication.
Nicholas J Roberts et al.
Plant physiology, 161(1), 556-567 (2012-11-09)
Nodulation in legumes requires the recognition of rhizobially made Nod factors. Genetic studies have revealed that the perception of Nod factors involves LysM domain receptor-like kinases, while biochemical approaches have identified LECTIN NUCLEOTIDE PHOSPHOHYDROLASE (LNP) as a Nod factor-binding protein.
Visualizza tutti i documenti correlati

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.