A5477

Anticorpo monoclonale Anti-HA

clone HA-7, purified from hybridoma cell culture

• Sinonimo/i: Anti-HA, Anti-emoagglutinina dell’influenza
• Numero MDL: MFCD00803873
• Codice UNSPSC: 12352203
• NACRES: NA.56

Proprietà

• Origine biologica: mouse
• Coniugato: alkaline phosphatase conjugate
• Forma dell’anticorpo: purified from hybridoma cell culture
• Tipo di anticorpo: primary antibodies
• Clone: HA-7, monoclonal
• Forma fisica: buffered aqueous glycerol solution
• tecniche: western blot: 1:4,000 using extracts of mammalian cells expressing HA tagged fusion proteins
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Monoclonal anti-HA, Alkaline Phosphatase conjugate is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice, immunized with a synthetic peptide. Human influenza hemagglutinin (HA), a surface glycoprotein exists as a trimer. The monomeric HA comprises base and membrane distal-tip regions with conserved residues and receptor-binding site, respectively.

Specificità

L′anticorpo riconosce le forme native come quelle denaturate-ridotte delle proteine marcate con HA ed è reattivo con le proteine di fusione marcate con HA N- o C-terminale in E. coli o in cellule di mammifero.

Immunogeno

peptide sintetico corrispondente a un frammento dell′emoagglutinina (HA) del virus dell′influenza umana noto come HA-tag, coniugato con KLH

Applicazioni

Monoclonal Anti-HA−Alkaline Phosphatase antibody produced in mouse has been used in immunoblotting

Azioni biochim/fisiol

Human influenza hemagglutinin (HA) is required for the infectivity of the human virus. HA interacts with the host cell surface glycoproteins especially the sialic acid to initiate infection. The short sequence derived from the HA molecule corresponding to amino acids 98-106 (YPYDVPDYA) has been used as a tag, known as HA-Tag. This tag facilitates the detection, isolation, purification, coprecipitation of proteins and immunostaining proteins. Caspase 3/7 effectively cleaves HA-tag. Many recombinant proteins or protein of interest have been engineered to express the HA tag fusion protein. The HA-tag does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.

Stato fisico

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% BSA, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide as a preservative

Stoccaggio e stabilità

For continuous use and extended storage, store at 2-8 °C. Do not freeze. Working dilution samples should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids