A3854
Anti-β-actina monoclonale
clone AC-15, purified from hybridoma cell culture
Sinonimo/i:
Monoclonal Anti-β-Actin, Loading Control
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About This Item
Codice UNSPSC:
12352203
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
peroxidase conjugate
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
AC-15, monoclonal
Forma fisica
buffered aqueous solution
PM
antigen 42 kDa
Reattività contro le specie
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
Non deve reagire con
Dictyostelium discoideum
Drosophila
tecniche
western blot: 1:25,000-1:50,000 using cell extracts of human foreskin fibroblasts or chicken fibroblasts
Isotipo
IgG1
N° accesso UniProt
applicazioni
research pathology
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
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Descrizione generale
Nella colorazione delle criosezioni di tessuti ultrasottili di ventrigli di pollo, l′anticorpo marca i corpi densi, i canali longitudinali che collegano i corpi densi consecutivi che sono occupati anche dalla desmina, e la placca densa associata alla membrana. Non colora i muscoli cardiaci e scheletrici adulti, tranne che per alcune tracce che sono dovute alla contaminazione del campione con cellule non muscolari o che possono comparire se si utilizzano tessuti embrionali.
Actin is one of the most conserved eukaryotic proteins and is expressed in mammals and birds as at least six isoforms. There are three α-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-non muscle). Actin isoforms show >90% overall sequence homology, but only 50–60% homology in their NH2-terminal residues. ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. The ACTB gene is located on human chromosome 7p22.1.
Mouse monoclonal anti-β-actin-peroxidase antibody specifically localizes β-actin in a wide variety of tissues and species using immunoblotting (42kDa). The antibody cross-reacts with β-Actin expressed in cells of human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, and Hirudo medicinalis (leech) tissues, but not in Dictyostelium discoideum amoebae or Drosophila.
Mouse monoclonal anti-β-actin-peroxidase antibody specifically localizes β-actin in a wide variety of tissues and species using immunoblotting (42kDa). The antibody cross-reacts with β-Actin expressed in cells of human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, and Hirudo medicinalis (leech) tissues, but not in Dictyostelium discoideum amoebae or Drosophila.
Mouse monoclonal anti-β-actin-peroxidase antibody specifically localizes β-actin in a wide variety of tissues and species using immunoblotting (42kDa). The antibody cross-reacts with β-Actin expressed in cells of human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, and Hirudo medicinalis (leech) tissues, but not in Dictyostelium discoideum amoebae or Drosophila.
Immunogeno
peptide N-terminale lievemente modificato della β-actina citoplasmatica, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, coniugato a KLH.
Applicazioni
L′anti-actina murina monoclonale è stata utilizzata come controllo di caricamento per l′analisi con metodica Western blot di lisati di proteine epatiche di ratto.
L′anticorpo anti-actina murino monoclonale è stato utilizzato come controllo di caricamento per l′analisi con metodica Western blot di proteine immunoprecipitate da co-colture di gangli della radice dorsale di ratto.
Monoclonal mouse anti-beta actin antibody is suitable as a loading control or primary antibody for western blot analysis. It has also been used in chemiluminescent detection.
Azioni biochim/fisiol
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The six isoforms of actins are characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. β-Actin (ACTB) modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Stoccaggio e stabilità
For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Altre note
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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