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08124

Sigma-Aldrich

Isoamylase

ammonium sulfate suspension, ≥10,000,000 units/mg protein

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About This Item

Classificazione EC (Enzyme Commission):
3.2.1.68 (BRENDA, IUBMB)
Numero MDL:
MFCD00131418
Codice UNSPSC:
12352204
NACRES:
NA.54

Forma fisica

ammonium sulfate suspension

Attività specifica

≥10,000,000 units/mg protein

Temperatura di conservazione

2-8°C

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Applicazioni

Isoamylase from Pseudomonas sp. has been used in starch debranching and to measure the chain-length distributions.

Azioni biochim/fisiol

Pseudomonas amyloderamosa is considered a popular source of isoamylase. In the presence of a surfactant, this isoamylase produces a series of maltooligosaccharides. Immobilized isoamylase makes it applicable with a polysaccharide matrix composed of agarose, cellulose, and raw corn starch.
Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Definizione di unità

One unit causes an increase in A610 of 0.1 in 1 hour using a homogenized solution of rice starch as a substrate (pH 3.5, 40°C)

Risultati analitici

≥10 MU/mg protein

Pittogrammi

Skull and crossbonesEnvironment
GHS06,GHS09

Avvertenze

Danger

Indicazioni di pericolo

H302 + H332,H311,H411

Classi di pericolo

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2

Codice della classe di stoccaggio

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Sigma-Aldrich

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Amiloglucosidasi ≥260 U/mL, aqueous solution

Sigma-Aldrich

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Pullulan Standard Set Mp ~350 - 700′000 set of analytical standards, for GPC

Supelco

96351

Pullulan Standard Set Mp ~350 - 700′000

Amylose from potato used as amylase substrate

Sigma-Aldrich

A0512

Amylose from potato

Amyloglucosidase from Rhizopus sp. ≥40,000 units/g solid

Sigma-Aldrich

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Amyloglucosidase from Rhizopus sp.

β-Amylase from sweet potato Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)

Sigma-Aldrich

A7005

β-Amylase from sweet potato

Digestion properties of corn starch modified by $\alpha$-D-glucan branching enzyme and cyclodextrin glycosyltransferase
Li Y, et al.
Food Hydrocolloids, 89, 534-541 (2019)
Lucio Gullo et al.
Pancreas, 38(4), 396-400 (2009-03-20)
To determine the type and frequency of pancreatic lesions detected by magnetic resonance cholangiopancreatography (MRCP) in subjects with asymptomatic pancreatic hyperenzymemia and to assess for a possible relationship between these lesions and the hyperenzymemia. From January 2005 to May 2008
Sebastian Streb et al.
The Plant cell, 20(12), 3448-3466 (2008-12-17)
Several studies have suggested that debranching enzymes (DBEs) are involved in the biosynthesis of amylopectin, the major constituent of starch granules. Our systematic analysis of all DBE mutants of Arabidopsis thaliana demonstrates that when any DBE activity remains, starch granules
Hiroki Takata et al.
Carbohydrate research, 344(5), 654-659 (2009-02-17)
Glycogen, highly branched (1-->4)(1-->6)-linked alpha-d-glucan, can be extracted from natural sources such as animal tissues or shellfish (natural source glycogen, NSG). Glycogen can also be synthesized in vitro from glucose-1-phosphate using the cooperative action of alpha-glucan phosphorylase (GP, EC 2.4.1.1)
Hongjie An et al.
Journal of food science, 76(1), N11-N14 (2011-05-04)
Starch debranching is fundamental for understanding the structure-function relationships of starch. In this paper, atomic force microscopy (AFM) was used to investigate potato starch by isoamylase [EC 3.2.1.68] debranching at nanometer scale. The hydrolysates were separated by gel-permeation chromatography and
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