B9431

Biotin-4-Fluorescein

≥95% purity (HPLC), powder

• Sinonimo/i: 5-(6)-[2-(biotinoyl)aminoethylaminocarbonyl] fluorescein, B4F, Biotin (5-fluorescein) conjugate
• Formula empirica (notazione di Hill): C₃₃H₃₂N₄O₈S
• Peso molecolare: 644.69
• Numero MDL: MFCD09264223
• Codice UNSPSC: 12171500
• eCl@ss: 34058011
• ID PubChem: 329773319
• NACRES: NA.47

Proprietà

• Nome del prodotto: Biotin-4-Fluorescein, ≥95.0% (HPLC)
• Livello qualitativo: 100; 200
• Saggio: ≥95.0% (HPLC)
• Stato: powder
• PM: 644.71
• tecniche: titration: suitable
• Colore: light orange to very dark orange; light yellow to very dark yellow
• ε (coefficiente d’estinzione): ≥70 at 491-497 nm in phosphate buffer at 0.05 M
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: room temp
• Stringa SMILE: OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56
• InChI: 1S/C33H32N4O8S/c38-18-7-10-25-23(14-18)29(21-9-6-19(39)15-26(21)45-25)20-8-5-17(13-22(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30; UROXVNHIYQXTRA-LFERIPGTSA-N

Descrizione

Applicazioni

Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin.

Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin.

Azioni biochim/fisiol

Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)