B0287
Monoclonal Anti-FITC−Biotin antibody produced in mouse
clone FL-D6, purified immunoglobulin, buffered aqueous solution
Sinonimo/i:
Monoclonal Anti-FITC
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About This Item
Codice UNSPSC:
12352203
NACRES:
NA.43
Prodotti consigliati
Origine biologica
mouse
Coniugato
biotin conjugate
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
FL-D6, monoclonal
Forma fisica
buffered aqueous solution
tecniche
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil
Isotipo
IgG1
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
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Descrizione generale
Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Specificità
The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).
Immunogeno
FITC-BSA conjugate
Applicazioni
Monoclonal Anti-FITC-Biotin antibody produced in mouse has been used:
- for amplification of signal in immunofluorescence assays
- in Ag-activated beads for flow-based micro immunoassay of parathyroid hormone and IL-5
- enzyme linked immunosorbent assay (ELISA)
Azioni biochim/fisiol
Fluorochrome labeling provides a rapid and accurate localization of the site of antigen-antibody interaction when one of the reactants from parts of a cell, tissue or other biological structure. Fluorescein isothiocyanate (FITC) is a commonly used marker for antibodies in immunofluorescence techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labelled substances. FITC is widely used as a hapten to label different proteins and anti-FITC antibodies are used to identify these labeled proteins. Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent, such as alkaline phosphatase, horseradish peroxidase or biotin.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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M A Hayes et al.
Analytical chemistry, 73(24), 5896-5902 (2002-01-17)
A small-volume heterogeneous immunoassay system is demonstrated in microchannels exploiting magnetic manipulations of small paramagnetic particles (1-2-microm diameter). The small-diameter particles help to create a high surface-area-to-volume ratio that generates the sensitivity for the small detection volumes. Flow characteristics of
Flow-based microimmunoassay
Hayes MA, et al.
Analytical Chemistry, 73(24), 5896-5902 (2001)
I J Harmer et al.
Journal of immunological methods, 122(1), 115-121 (1989-08-15)
In a model ELISA system alkaline phosphatase (AP) absorbed onto microtitre wells was employed as the target antigen. The antigen was then reacted with a monoclonal antibody to AP either unlabelled or labelled with (a) FITC and (b) biotin. The
Ali Gorgin Karaji et al.
International immunopharmacology, 5(6), 1019-1027 (2005-04-15)
Opioid peptides modulate immune responses via ligation to classical opioid receptors (mu, delta and kappa), expressed on immune cells, or in an indirect fashion via the central nervous system. The combination of immunofluorescent technique and flow cytometry has proven to
Chapter 11 - (Strept)avidin?Biotin Systems
Bioconjugate Techniques, 465-505 (2013)
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