B0126

Bromophenol Blue

Powder

• Sinonimo/i: 3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein, Bromophenol Blue (BPB), Bromphenol Blue Sultone Form
• Formula empirica (notazione di Hill): C₁₉H₁₀Br₄O₅S
• Numero CAS: 115-39-9
• Peso molecolare: 669.96
• Beilstein: 61698
• Numero CE: 204-086-2
• Numero MDL: MFCD00005875
• Codice UNSPSC: 12171500
• ID PubChem: 24891504
• NACRES: NA.47

Proprietà

• product name: Bromophenol Blue, titration: suitable
• agenzia: suitable for SM 2310; suitable for SM 2320
• Livello qualitativo: 200
• Forma fisica: powder
• tecniche: titration: suitable
• Colore: faint beige to very dark beige; faint orange to very dark orange
• visual transition interval: 3.0-4.6, yellow-green to blue
• Punto di fusione: 273 °C
• Solubilità: methanol: 10 mg/mL
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: room temp
• Stringa SMILE: Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
• InChI: 1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H; UDSAIICHUKSCKT-UHFFFAOYSA-N

Descrizione

Descrizione generale

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Applicazioni

Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)