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114413

Sigma-Aldrich

Bromothymol Blue

ACS reagent, Dye content 95 %

Sinonimo/i:

3′,3″-Dibromothymolsulfonphthalein

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About This Item

Formula empirica (notazione di Hill):
C27H28Br2O5S
Numero CAS:
76-59-5
Peso molecolare:
624.38
Beilstein:
373934
Numero CE:
200-971-2
Numero MDL:
MFCD00005872
Codice UNSPSC:
12171500
ID PubChem:
24847171
NACRES:
NA.47

Grado

ACS reagent

Livello qualitativo

200

Stato

powder

Composizione

Dye content, 95%

Colore

pink-purple

visual transition interval

6.0-7.6, yellow to blue(passes test)

Punto di fusione

200-202 °C ((392 - 396 °F) - lit.)
200-202 °C (lit.)

λmax

420 nm

ε (coefficiente d’estinzione)

≥17000 at 419-425 nm in methanol
≥8000 at 326-332 nm in methanol
≥9000 at 279-285 nm in methanol

Temperatura di conservazione

room temp

Stringa SMILE

CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C

InChI

1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3
NUHCTOLBWMJMLX-UHFFFAOYSA-N

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Descrizione generale

Bromothymol Blue, also known as, dibromothymolsulfonphthalein is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthalein. It is a pH-sensitive dye that changes color from yellow to blue.

Applicazioni

Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include:
  • as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
  • for the demonstration of fungal hyphae within plant roots
  • in the assessment of the soluble acidity within single rice grains
  • use in an agar gel medium for enumeration of Bacillus cereus
  • as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
SHBN5829
MKCK0744
MKCG9344
MKCG4045
MKCC4196

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