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Key Documents

95290

Sigma-Aldrich

Methyl Blue

for microscopy (Bot., Hist.), indicator (pH 9.4-14.0)

Sinonimo/i:

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

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About This Item

Formula empirica (notazione di Hill):
C37H27N3Na2O9S3
Numero CAS:
Peso molecolare:
799.80
Colour Index:
42780
Numero CE:
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
MA.02

Grado

for microscopy (Bot., Hist.)
indicator (pH 9.4-14.0)

Livello qualitativo

Forma fisica

powder or crystals

pH

9.4-14

Solubilità

water: 0.01 g/10 mL, blue to very deep blue

εmax

≥200 at 310-320 nm
≥400 at 595-605 nm

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2
MCPLVIGCWWTHFH-UHFFFAOYSA-L

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Categorie correlate

Descrizione generale

Methyl Blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.

Applicazioni

Methyl Blue has been used:
  • to stain leaf disks for callose visualization within the cells
  • to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
  • as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
  • in Mann′s stain as it involves a methyl blue-eosin mixture

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

572.0 °F - Pensky-Martens closed cup

Punto d’infiammabilità (°C)

> 300 °C - Pensky-Martens closed cup

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves


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A novel flow injection method with resonance light scattering detection was developed for the determination of total protein concentrations. This method is based on the enhancement of RLS signals from Methyl Blue (MB) by protein. The enhanced RLS intensities at

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