51558

HEPES buffered saline

BioUltra, for molecular biology, 2× concentrate

• Sinonimo/i: HEPES Saline Buffer Solution
• Numero MDL: MFCD00006158
• Codice UNSPSC: 12161700
• ID PubChem: 329757705
• NACRES: NA.25

Proprietà

• Grado: for molecular biology
• Livello qualitativo: 200
• Nome Commerciale: BioUltra
• Forma fisica: liquid
• Qualità: 2× concentrate
• Composizione: dextrose, 2.0 g/L ; HEPES, 10 g/L ; KCl, 0.74 g/L ; NaCl, 16 g/L ; Na₂HPO₄.2H₂O, 0.27 g/L
• Concentrazione: 2 ×
• Impurezze: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• Indice di rifrazione: n20/D 1.339
• pH: 7.2 (25 °C)
• Cationi in tracce: Al: ≤1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: neat
• Assorbanza UV: λ: 260 nm A_max: 0.06; λ: 280 nm A_max: 0.04
• Stringa SMILE: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14); JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descrizione

Descrizione generale

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Applicazioni

HEPES buffered saline has been used:

• for the preparation of plasmid DNA
• as a reagent to prepare transfection master mix for DNA construct vectors
• to develop hydrogel precursor for cell leakage assay

Componenti

concentration (in H₂O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na₂HPO₄.2H₂O; 2.0 g/l dextrose

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves