EHIFIDNTP-RO

Expand^™ High Fidelity PCR System, dNTPack

suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included

• Codice UNSPSC: 41106300
• NACRES: NA.28

Proprietà

• impiego: sufficient for ≤1,000 reactions (04738276001); sufficient for ≤200 reactions (04738268001); sufficient for ≤40 reactions (04738250001)
• Livello qualitativo: 100
• Caratteristiche: High Fidelity PCR; dNTPs included; hotstart: no
• Confezionamento: pkg of 2,500 U (04738276001 [10x250 U]); pkg of 100 U (04738250001); pkg of 500 U (04738268001 [2x250 U])
• Produttore/marchio commerciale: Roche
• tecniche: PCR: suitable
• input: purified DNA
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Expand High Fidelity PCR System is optimized to efficiently amplify DNA fragments up to 5 kb. The Expand High Fidelity PCR System is composed of a special enzyme mix containing thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. The fidelity of DNA synthesis with Expand High Fidelity PCR System shows a threefold increase compared to Taq DNA Polymerase. T/A cloning is recommended.

Applicazioni

The Expand^™ High Fidelity PCR System is an enzyme blend delivering superior results with twofold higher yield and threefold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length for:

• PCR
• RT-PCR

For maximum convenience, select the 2x concentrated ready-to-use High Fidelity Master.

Caratteristiche e vantaggi

• Use one enzyme for all applications. Enjoy high yield, fidelity, and flexibility.
• Detect PCR products previously undetectable and avoid false negatives.
• Achieve successful results from small quantities of template DNA.

Confezionamento

1 kit containing 5 components

Qualità

Each lot of Expand High Fidelity is function-tested in PCR using human genomic DNA and specific primers for collagen (1kb amplified product) and the tPA gene (4.8kb amplified product).

Definizione di unità

Volume Activity: 3.5U/μL
Each dNTPack contains 10mM additive-free sodium salt nucleotides in a ready-to-use mix.

Altre note

For life science research only. Not for use in diagnostic procedures.

The 5′→3′ polymerase activity of Taq DNA Polymerase and the 3′→5′ exonuclease of the proofreading polymerase combine for improved performance and results; 3′→5′ exonuclease proofreading produces a threefold increase in DNA synthesis fidelity compared to Taq DNA polymerase.

Note legali

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Expand is a trademark of Roche

Solo come componenti del kit

• Expand High Fidelity Enzyme mix
• Expand High Fidelity Buffer, with 15 mM MgCl₂ 10x concentrated
• Expand High Fidelity Buffer, without MgCl₂
• MgCl₂ Stock Solution 25 mM
• PCR Nucleotide Mix

Informazioni sulla sicurezza

• Indicazioni di pericolo: H412
• Consigli di prudenza: P273 - P501
• Classi di pericolo: Aquatic Chronic 3
• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash