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DIGUTP-RO

Roche

Digoxigenin-11-UTP

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About This Item

Codice UNSPSC:
41105500
NACRES:
NA.21

Saggio

≥85% (HPLC)

Livello qualitativo

100

Forma fisica

solution

PM

(Mr = 1106.7 (DIG-11-UTP-Li4))

Confezionamento

pkg of 25 μL (11209256910 [10 mM])
pkg of 57 μL (03359247910 [3.5 mM])

Produttore/marchio commerciale

Roche

Temperatura di conservazione

−20°C

Descrizione generale

Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.

Applicazioni

Digoxigenin-11-UTP has been used for labeling RNA probes in:
  • in situ hybridization
  • in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
  • fluorescence in situ hybridization (FISH)

Azioni biochim/fisiol

Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.

Qualità

Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.

Formula: C43H61N4O22P3Li4

Altre note

For life science research only. Not for use in diagnostic procedures.

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H302

Classi di pericolo

Acute Tox. 4 Oral

Codice della classe di stoccaggio

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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