11684302001
Roche
ABTS™ Solution
solution (ready-to-use), suitable for ELISA
Sinonimo/i:
ABTS™ Solution, ABTS
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About This Item
Codice UNSPSC:
12352204
Prodotti consigliati
Forma fisica
solution (ready-to-use)
Livello qualitativo
impiego
sufficient for 1,500-3,000 assays (ELISA)
Confezionamento
pkg of 3 × 100 mL
Produttore/marchio commerciale
Roche
tecniche
ELISA: suitable
Temperatura di conservazione
2-8°C
Descrizione generale
ABTS™ Solution is a ready to use substrate for peroxidase-driven indicator reactions. It contains 2,2-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and H2O2 in glycin/citric acid buffer and is available in slightly green color.
Applicazioni
ABTS Solution is a chromogenic substrate for peroxidase in ELISA assays.
Azioni biochim/fisiol
2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS™ acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS™ is a highly sensitive, specific and reproducible technique This solution is an excellent substrate for enzyme immunoassays with horseradish peroxidase as a marker enzyme.
Stato fisico
Reaction product:
Color: green
Evaluation: photometric (405nm)
Color: green
Evaluation: photometric (405nm)
Risultati analitici
Absorption: ABTS absorption spectrum reference must always be measured and is determined by a spectrum. The Reference wavelength of 490 nm or higher should be selected due to no measureable absorption. The Reference absorption is usually automatically subtracted by the Plate Reader (background).
Altre note
For life science research only. Not for use in diagnostic procedures.
Note legali
ABTS is a trademark of Roche
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
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A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as a substrate for horseradish peroxidase (HRP) conjugate was studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for
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