11582950001
Roche
BM Chemiluminescence ELISA Substrate (POD)
Sinonimo/i:
ELISA substrate
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About This Item
Codice UNSPSC:
12352204
Prodotti consigliati
![BM Chemiluminescence Western Blotting Substrate (POD) pkg of 1 set (11500708001 [1,000 cm2 membrane]), pkg of 1 set (11500694001 [4,000 cm2 membrane])](/deepweb/assets/sigmaaldrich/product/images/352/091/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1/640/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1.jpg)
Stato
solution
Livello qualitativo
Confezionamento
pkg of 250 mL (for 2500 wells or 1000 tubes)
Produttore/marchio commerciale
Roche
Condizioni di stoccaggio
(Keep container tightly closed in a dry and well-ventilated place.)
tecniche
ELISA: suitable
Colore
clear colorless
Solubilità
water: miscible
Compatibilità
suitable for chemiluminescence
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
The sensitivity of enzyme-linked immunosorbent assays often depends on the detection limit of the colorimetric substrates used. In addition, the dynamic range of a colorimetric assay is restricted to a maximum of two orders of magnitude due to the physical/chemical limitations of absorbance measurement (Law of Lambert & Beer). With chemiluminescence technology, a nonradioactive method, which combines the convenience of tube or microplate-based immunoassays with the advantages of isotopic assays, is now available. The BM Chemiluminescence ELISA Substrate (POD) has been evaluated with different immunoassays on the LB 96 P microplate reader and the LB 953 tube chemiluminescence analyzer from EG&G;Berthold.
Applicazioni
The BM Chemiluminescence ELISA Substrate (POD) provides a substrate solution for peroxidase-based (POD, HRP) secondary detection systems with highly sensitive, enhanced chemiluminescence. This reagent has been optimized for ELISA applications to be run on a microplate chemiluminescence reader (96-well format) or tube-format luminometers.
Caratteristiche e vantaggi
- Improved sensitivity
- Large dynamic range
- Rapid and constant signal
Contents
Substrate Reagent A (buffered solution that contains luminol/4-iodophenol)
Starting Reagent B (buffered solution that contains a stabilized form of H2O2)
Principio
Horseradish peroxidase (POD, HRP), in the presence of hydrogen peroxide (H2O2), catalyzes the oxidation of diacylhydrazides such as luminol. A reaction product in an excited state is thus formed, then decays to the ground state by emitting light. Strong enhancement of the light emission is achieved by the agent 4-iodophenol (contained), which acts as a radical transmitter between the formed oxygen radical and luminol.
Nota sulla preparazione
Working solution: Preparation of working solution
The BM Chemiluminescence ELISA Substrate is supplied as a set of two stable solutions. Depending on the scale of the assay, the appropriate amount of substrate solution has to be prepared 15 minutes before use:
Storage conditions (working solution): The premixed solution is stable for 1 week, when stored at 2 to 8 °C.
The BM Chemiluminescence ELISA Substrate is supplied as a set of two stable solutions. Depending on the scale of the assay, the appropriate amount of substrate solution has to be prepared 15 minutes before use:
- Add to 100 parts of solution A one part of solution B.
- Stir the mixture for at least 15 minutes at 15 to 25 °C to equilibrate the components.
Storage conditions (working solution): The premixed solution is stable for 1 week, when stored at 2 to 8 °C.
Altre note
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
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