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11669915001

Roche

Cell Proliferation ELISA, BrdU (chemiluminescent)

Sinonimo/i:

BrdU, cell proliferation

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About This Item

Codice UNSPSC:
41116158

impiego

sufficient for ≤1,000 tests

Livello qualitativo

100

Produttore/marchio commerciale

Roche

tecniche

ELISA: suitable

Metodo di rivelazione

chemiluminescent

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

Chemiluminescent immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis:  a nonradioactive alternative to the [3H]-thymidine incorporation assay.

Specificità

The antibody conjugate reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.

Applicazioni

Cell Proliferation ELISA, BrdU (chemiluminescent) has been used:
  • for bromodeoxyuridine (BrdU) proliferation assay in ReN and primary astrocytes
  • to test the effect on imatinib on the proliferation of neuroblastoma cells using BrdU incorporation assay
  • to check the effect of palbociclib on cell cycle inhibition using BrdU incorporation in pancreatic patient-derived primary cell lines

The Cell Proliferation ELISA, BrdU (chemiluminescent) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple nonradioactive alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:
  • Detection and quantification of cell proliferation induced by growth factors and cytokines
  • Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
  • Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
  • Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research

Confezionamento

1 kit containing 7 components.

Componenti

  1. BrdU Labeling Reagent, 1,000x conc
  2. FixDenat
  3. Anti-BrdU-POD 4. Antibody Dilution Solution
  4. Washing Buffer PBS, 10x conc.
  5. Substrate Component A
  6. Substrate Component B

Altre note

For life science research only. Not for use in diagnostic procedures.

Solo come componenti del kit

N° Catalogo
Descrizione
  • BrdU Labeling Reagent
  • FixDenat ready-to-use
  • Anti-BrdU-peroxidase antibody
  • Antibody Dilution Solution ready-to-use
  • ready-to-use 10x concentrated
  • Substrate Component A
  • Substrate Component B

Avvertenze

Danger

Indicazioni di pericolo

H225,H317,H319,H340

Classi di pericolo

Eye Irrit. 2 - Flam. Liq. 2 - Muta. 1B - Skin Sens. 1

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Cell Cycle Analysis Using a Nucleoside Triphosphate (NTP) Transporter Molecule for Rapid DNA Labeling in Living Cells

Regulation of the cell cycle involves processes crucial to the survival of a cell, including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division associated with cancer. The cell cycle is a four-stage process in which the cell 1) increases in size (G1-stage), 2) copies its DNA (synthesis, S-stage), 3) prepares to divide (G2-stage), and 4) divides (mitosis, M-stage). Due to their anionic nature, nucleoside triphosphates (NTPs), the building blocks of both RNA and DNA, do not permeate cell membranes.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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