11088939001

Tumor Necrosis Factor-α, human (hTNF-α)

recombinant (yeast)

• Sinonimo/i: hTNF, human necrosis factor
• Codice UNSPSC: 12352207

Proprietà

• Origine biologica: human
• Livello qualitativo: 100
• Ricombinante: expressed in yeast
• Saggio: ≥95% (SDS-PAGE)
• Forma fisica: solution
• Potenza: ≤0.01 ng/mL EC₅₀
• Attività specifica: >1*10^8 U/mg (>1 x 10^⊥8 U/mg, <0.01 ng/ml (hTNF-a, NIBSC, interim standard, 87/650), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.)
• PM: 17,000 Da
• Confezionamento: pkg of 1,000,000 U (10 μg, 1ml)
• Produttore/marchio commerciale: Roche
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• Impurezze: <0.1 EU/μgtested (LAL test)
• N° accesso UniProt: Q9UBM5
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... TNF(7124)

Descrizione

Descrizione generale

Tumor necrosis factor a (TNF-a) is a multifunctional cytokine, majorly produced by activated macrophages and is involved in the pathogenesis of many acute and chronic inflammatory condition.

Recombinant, Tumor Necrosis Factor-α, human (hTNF-α), is produced in Pichia pastoris and purified by standard chromatographic techniques.

Primary structure: One polypeptide chain (157 amino acids), identical to natural hTNF-a.

Specificità

Human hTNF-α is effective on human and mouse cells.

Species specificity: human, mouse

Applicazioni

Tumor Necrosis Factor-α (hTNF-α)

• Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines.
• Has activating and growth stimulating activities on a variety of normal cells.
• Has antiviral activity on many cell types in vitro.
• Can cause tumor necrosis of certain tumors in vivo.

Azioni biochim/fisiol

Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.

Qualità

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

Sequenza

Chain Length 157 AA

One polypeptide chain (157 amino acids), identical to natural hTNF-α.

Definizione di unità

EC₅₀ definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml).

Stato fisico

Solution, filtered through 0.2 μm pore size membrane.

Nota sulla preparazione

Working concentration: 1 ng/ml
For complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.
Working solution: Dilute the concentrated hTNF-α solution (10 μg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.
Storage conditions (working solution): -15 to -25 °C
It is recommended to store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Altre note

For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash