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SCT012M

Sigma-Aldrich

LuminiCell Tracker 540- Vascular Labeling Kit

Biocompatible organic fluorescent nanoparticles (AIEDots) that can be used to fluorescently tag vasculature in living tissues and animals for studies of inflammation and vascular leakage.

Sinonimo/i:

AIEDots Cell Tracker Dye, AIE Live Cell Fluorescent Dye, Fluorescent Cell Tracking Dye, Live Cell Fluorescent Nanoparticles

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352207
eCl@ss:
32161000
NACRES:
NA.47

Livello qualitativo

tecniche

cell culture | mammalian: suitable

Metodo di rivelazione

fluorometric

Descrizione generale

Absorbance Maximum: 423nm
Emisson Maximum: 540nm

Suggested Filters:
Excitation: 405/458/488nm
Emisson: 480-560nm
Long-term noninvasive cell tracking by fluorescent probes and quantum dots is of great importance to life science and biomedical engineering. Current methods used to fluorescently tag cells have been limited by short signal duration, high background auto-fluorescence or lengthy molecular cloning manipulations using GFP. LuminiCell Trackers<TMSYMBOL></TMSYMBOL> are biocompatible organic fluorescent nanoparticles based on Aggregation Induced Emission technology, termed AIEDots. Aggregation induced emission (AIE) molecules emit fluorescence in an opposite manner than other common fluorophores (Quantum Dots, GFP). Propeller-shaped AIE fluorogens are non-emissive in solutions but become highly fluorescent upon aggregate formation. Due to these differences, LuminiCell Trackers<TMSYMBOL></TMSYMBOL> have very high fluorescence intensities with minimal signal quenching allowing live cell fluorescent tagging for up to 10 days in vitro and 21 days in vivo. These properties make them optimal candidates for long interval live cell bioimaging experiments.

The Luminicell Tracker<TMSYMBOL></TMSYMBOL>-540 Vascular Labeling Kit contain green fluorescent AIEDots nanoparticles without TAT sequences. These nanoparticles can be used to fluorescently tag vasculature in living tissues and animals for studies of inflammation and vascular leakage.

Features and Benefits

● Brighter: 10X brighter than other cell labeling technologies
● Photostable: 3X longer fluorescence without signal quenching
● Biocompatible: Non-toxic organic nanoparticles intended for biological applications
● Rapid Protocol: Easy-to-use protocol labels cells within 4 hours

Applicazioni

Biocompatible organic fluorescent nanoparticles (AIEDots) that can be used to fluorescently tag vasculature in living tissues and animals for studies of inflammation and vascular leakage.

Qualità

Absorbance: 422+-5nM
Concentration: 180-220nM
Fluorescence: 540+/- 10nm
Quantum Yield: ≥50%
Brightness at 540nM: ≥3X10^7 M-1cm-1

Stato fisico

500nM in 0.5ml PBS, pH 7.4

Stoccaggio e stabilità

Store at -2-8°C upon receipt. Thaw at room temperature or in a water bath. Do not Freeze.

Note: Some particulates may form as a result of nanoparticle aggregation during shipping. To get particulates back in solution, sonicate the vial containing LuminiCell Tracker three times for 1 min each before use.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Shiying Liu et al.
ACS applied materials & interfaces, 8(19), 11925-11933 (2016-04-29)
Mesenchymal stem cells (MSCs) have proved to be a promising and abundant cell source for tissue and organ repair in regenerative medicine. However, the cell fate, distribution and migration of these transplanted cells are still unclear due to the limited

Articoli

Biocompatible organic fluorescent nanoparticles (AIE Dots) that can be used to label cells and vasculature for long-term live cell tracking and tracing experiments.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.