SCC116
GT1-7 Mouse Hypothalamic GnRH Neuronal Cell Line
Mouse
Sinonimo/i:
GT1-7 hypothalamic cell line, GT1-7 neural cell line
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About This Item
Codice UNSPSC:
41106514
eCl@ss:
32011203
NACRES:
NA.81
Prodotti consigliati
product name
GT1-7 Mouse Hypothalamic GnRH Neuronal Cell Line, GT1-7 cells can be used as an in vitro model of GnRH-secreting neurons of the hypothalamus.
Origine biologica
mouse
Livello qualitativo
tecniche
cell based assay: suitable
cell culture | mammalian: suitable
Condizioni di spedizione
ambient
Descrizione generale
GT1-7 is an immortalized mature mouse hypothalamic GnRH neuronal cell line. Immortalized GnRH neurons were generated by introducing a transgene containing the promotor region of the GnRH gene coupled to the coding region of the SV40 T-antigen oncogene into transgenic mice1. The resulting anterior hypothalamic tumors were removed from one of the mice and the cells dissociated and cloned. GT1-7 is a clonal line of mature differentiated GnRH neurons that exhibit high levels of Gnrh1 mRNA and secrete GnRH in response to depolarization.
Gonadotrophin-releasing hormone (GnRH) is a neuropeptide that is synthesized and released from GnRH neurons within the hypothalamus and is required for normal reproductive development and function. The scarcity and scattered distribution of GnRH neurons in the rostral hypothalamus make the biological study of the cells difficult.
Descrizione della linea cellulare
Neural Lineage Cells
Applicazioni
Research Category
Endocrine
Neuroscience
Endocrine
Neuroscience
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
Qualità
• Each vial contains ≥ 1X106 viable cells.
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination.
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination.
Stoccaggio e stabilità
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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W C Wetsel et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(9), 4149-4153 (1992-05-01)
Mammalian reproduction is dependent upon intermittent delivery of luteinizing hormone-releasing hormone (LHRH) to the anterior pituitary. This mode of secretion is required to sensitize maximally the gonadotrophs to LHRH stimulation and to regulate gonadotropin gene expression. While LHRH secretion is
Z Liposits et al.
Endocrinology, 129(3), 1575-1583 (1991-09-01)
An immortalized LHRH cell line has recently been developed by genetically targeting these neurons for tumorigenesis. One of the subclones, the GT1-7 cells, was characterized at both the light and electron microscopic levels to study the cellular and subcellular organization
Angela L Whittsette et al.
iScience, 25(8), 104754-104754 (2022-08-09)
The endoplasmic reticulum membrane complex (EMC) plays a critical role in the biogenesis of tail-anchored proteins and a subset of multi-pass membrane proteins in the endoplasmic reticulum (ER). However, because of nearly exclusive expression of neurotransmitter-gated ion channels in the
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