PC176
Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb
liquid, Calbiochem®
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About This Item
Codice UNSPSC:
12352203
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Stato
liquid
contiene
≤0.1% sodium azide as preservative
Reattività contro le specie
mouse, sheep, rat
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
avoid repeated freeze/thaw cycles
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
rat ... Htr2A(29595)
Descrizione generale
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
Recognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
This Anti-Serotonin 2A Receptor Rabbit pAb is validated for use in Frozen Sections, WB, Free Floating Sections, Paraffin Sections for the detection of Serotonin 2A Receptor.
Immunogeno
Rat
a synthetic peptide corresponding to amino acids 22-41 of rat 5-HT2A receptor
Applicazioni
Frozen Sections (1:300-1:500)
Immunoblotting (1:500-1:1000)
Free Floating Sections (see application references)
Paraffin Sections (see application references; heat pre-treatment required)
Attenzione
Toxicity: Standard Handling (A)
Stato fisico
In 150 mM NaCl, 20 mM sodium phosphate, 1% BSA, pH 7.5.
Ricostituzione
Following initial thaw, aliquot and freeze (-20°C).
Altre note
Antibody specificity was examined in the rat amygdala, cortex, and hippocampus. Detection of primary antibody with ABC according to manufacturers directions. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. Addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solutions allows further dilution of the primary antibody to approximately 1:1000 or greater. For staining paraffin sections, heating in 10 mM citrate buffer is required (see application references). Antibody should be titrated for optimal results in individual systems.
Leonard, B.E. 1996. Psychother. Psychosom.65, 66.
Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol.8, 175.
Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol.8, 175.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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Gongliang Zhang et al.
Neuropharmacology, 109, 332-340 (2016-04-27)
The rodent hippocampus supports non-spatial object memory. Serotonin 5-HT2A receptors (5-HT2AR) are widely expressed throughout the hippocampus. We previously demonstrated that the activation of 5-HT2ARs enhanced the strength of object memory assessed 24 h after a limited (i.e., weak memory) training
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Spaced training is robustly superior to massed training, which is a well-documented phenomenon in humans and animals. However, the mechanisms underlying the spacing effect still remain unclear. We have reported previously that spacing training exerts memory-enhancing effects by inhibiting forgetting
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