MABT110

Anti-Aggrecan (neoepitope) Antibody, clone BC-3

clone BC-3, from mouse

• Sinonimo/i: Aggrecan core protein, Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1, Aggrecan core protein 2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: BC-3, monoclonal
• Reattività contro le specie: bovine, human
• tecniche: immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_001126
• N° accesso UniProt: P16112
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ACAN(176)

Descrizione

Descrizione generale

Aggrecan (CSPG1, CSPCP, or CSPGCP) is a chondroitin-sulfate proteoglycan belonging to the family of letican proteins. Aggrecan is a large globar structure containing multiple domains including carbohydrate-recognition domains; complement binding protein domains; immunoglobulin domains; proteoglycan tandem repeats; and in some cases, EGF-like domains. It can be degraded by aggrecanase. The N-terminus of aggrecan binds ECM hyaluronan in the presence of the linking protein, and forms complex aggregates, whereas the C-terminus binds a number of other ECM proteins including tenascins, glycolipids, and fibulins. Aggrecan is a major component of cartilage tissue and it enables this tissue to withstand compression by maintaining the viso-elastic properties of cartilage. Aggrecan may also facilitate neural maturation and plasticity by contributing to the formation of perineuronal nets in the developing CNS.

Specificità

This antibody detectes the Args neoepitope sequence in aggrecanase-cleaved aggrecan (Tortella, M. D., et al. (1999). Science. 284(5420):1664-1666.).

Immunogeno

Epitope: Args neoepitope sequence

Linear peptide corresponding to human Aggrecan neoepitope.

Applicazioni

Anti-Aggrecan (neoepitope) Antibody, clone BC-3 detects level of Aggrecan (neoepitope) & has been published & validated for use in Western Blotting & IHC.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Western Blot Analysis: A representative lot detected Aggrecan neoepitope in human chondrocite cell lysate.

Qualità

Evaluated by Western Blot in SW1353 cell lysate.

Western Blot Analysis:1 µg/mL of this antibody detected Aggrecan neoepitope in 10 µg of SW1353 cell lysate.

Descrizione del bersaglio

~60 kDa observed. Uniprot describes three isoforms at ~250 kDa, ~246 kDa and ~239 kDa Aggrecan is degraded by aggrecanase into a ~60 kDa protein and other higher molecular weight products (Lemons, M. L. et al. (2001). J Neurosci. 21(13):4772-4781. Forsang, A. J. et al. (1995). Biochem J. 310(Pt 1):337-343.).

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
SW1353 cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable