MABS27

Anti-Diglycyl Lysine (Clone GX41) Antibody

clone GX41, from mouse

• Sinonimo/i: Diglycyl Lysine
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: GX41, monoclonal
• Reattività contro le specie: human, mouse, rat
• Reattività contro le specie (prevista in base all’omologia): all
• tecniche: immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Anti-Diglycyl Lysine has been shown to specifically detect peptides derived from the ubiquitinated portion of proteins. Diglycyl Lysine, clone GX41 is thought to find diglycyl-lysine moieties that are created and left behind at places of ubiquitination after digestion with trypsin has occurred.

Specificità

Antibody recognizes lysine residues modified by diglycine, a residue left at sites of ubiquitination after trypsin digestions.

Broad species cross-reactivity expected based on 100% sequence homology.

Immunogeno

Linear peptide corresponding to Diglycyl Lysine.

Applicazioni

Anti-Diglycyl Lysine (Clone GX41) Antibody detects level of Diglycyl Lysine (Clone GX41) & has been published & validated for use in WB & IP.

Immunoprecipitation Analysis: 10 µg of Anti-Diglycyl Lysine immunoprecipitated peptides containing Diglycyl Lysine modification from isolated ubiquitinated proteins which were obtained from a human cell line.

Mass Spectrometry Analysis: A previous lot was used by an independent a laboratory in Mass Spec. Image courtesy of Samie Jaffrey, Cornell Medical College.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Qualità

Evaluated by Western Blot in BSA protein and Diglycine modified BSA.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Diglycyl Lysine on 10 µg of BSA protein and Diglycine modified BSA.

Descrizione del bersaglio

Molecular weight can vary depending on the size of the protein or peptides containing Diglycyl modified lysine residues.

Stato fisico

Format: Purified

Protein G

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
BSA protein and Diglycine modified BSA

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable