MABS2253

Anti-LRP1 α-chain Antibody, clone 8G1

• Sinonimo/i: Anti-LRP1 Antibody
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 8G1, monoclonal
• PM: calculated mol wt 504.61 kDa
• Purificato mediante: using protein G
• Reattività contro le specie: human
• Confezionamento: antibody small pack of 100 μg
• tecniche: ELISA: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• Sequenza dell’epitopo: N-terminal
• N° accesso ID proteina: NP_002323
• N° accesso UniProt: Q07954
• Condizioni di spedizione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Prolow-density lipoprotein receptor-related protein 1 (UniProt Q07954; also known as A2MR, Alpha-2-macroglobulin receptor, APER, Apolipoprotein E receptor, CD19. LRP-1) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID: 4035) in human. LRP-1 is single pass type I membrane protein that is expressed in most tissues, but is abundant in liver, brain, and lung. It serves as a major VSMC receptor with a crucial role in maintaining vessel wall integrity and its expression in adult smooth muscle cells is shown to be essential for maintaining a contractile phenotype. LRP1 is synthesized with a signal peptide sequence (aa 1-19) and is processed in trans-Golgi network by Furin to generate a 515 kDa a subunit and an 85 kDa b subunit that contains the 100-amino intracellular domain (LRPICD; aa 4445-4544). The a and b subunits are non-covalently linked during LRP1 transport to the cell membrane. LRP1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (ApoE), APP and amyloid b. It is the primary receptor mediating transport of amyloid b peptides across the blood-brain barrier into circulation, thereby clearing them from the brain. LRP1 is required for early embryonic development and is involved in cellular lipid homeostasis and plasma clearance of chylomicron remnants and activated LRPAP1 (a2-macroglobulin). Excessive copper accumulation in the brain has been linked with reduced LRP1 mediated clearance of amyloid b peptides across the blood brain barrier, which may contribute to complications of Alzheimer′s disease.

Specificità

Clone 8G1 is a mouse monoclonal antibody that detects human LRP-1. It targets an epitope within the a chain in the extracellular domain.

Immunogeno

Full-length LRP-1 purified from human placenta and 515 kDa band excised from gel and used as immunogen.

Applicazioni

Quality Control Testing

Evaluated by Immunocytochemistry in MCF-7 cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LRP1 a-chain in MCF-7 cells.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected LRP1 -chain in Immunocytochemistry applications (Craig, J., et al. (2013). PLoS One. 8(7):e70432).

Immunoprecipitation Analysis: A representative lot immunoprecipitated LRP1 -chain in Immunoprecipitation applications (Ranganathan, S., et al. (2011). J. Biol. Chem.286(35):30535-30541; Boye, K., et al. (2017). Nat. Commun. 8(1):1571).

Western Blotting Analysis: A representative lot detected LRP1 -chain in Western Blotting applications (Strickland, D.K., et al. (1990). J Biol Chem.;265(29):17401-4; Yamamoto, K., et al. (2013). FASEB J.;27(2):511-21; Yamamoto, K. et al. (2016). Matrix Biol.;56:57-73; Boye, K., et al. (2017). Nat Commun.;8(1):1571).

ELISA Analysis: A representative lot detected LRP1 -chain in ELISA applications (Ranganathan, S., et al. (2011). J. Biol. Chem. 286(35):30535-3054).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-LRP1 a-chain, clone 8G1, Cat. No. MABS2253, is a mouse monoclonal antibody that detects LRP1 a -chain and is tested for use in ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Stato fisico

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable