MABE985

Anti-Fox1 Antibody, clone 1D10

clone 1D10, from mouse

• Sinonimo/i: RNA binding protein fox-1 homolog 1, Ataxin-2-binding protein 1, Fox-1 homolog A, Fox1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 1D10, monoclonal
• Reattività contro le specie: mouse
• tecniche: RIP: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_067452
• N° accesso UniProt: Q9JJ43
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Rbfox3(52897)

Descrizione

Descrizione generale

RNA binding protein fox-1 homolog 1 (UniProt Q9JJ43; also known as Ataxin-2-binding protein 1, Fox-1 homolog A, Hexaribonucleotide binding protein 1) is encoded by the Rbfox1 (also known as A2bp, A2bp1, Fox-1, Hrnbp1) gene (ORF MNCb-3035; Gene ID 268859) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Rbfox1 transcript itself is subjected to alternative splicing in response to chronic cell depolarization. This results in the increased expression of the nuclear, splicing-active Rbfox1 isoform, which in turn leads to increased splicing of Rbfox1 target transcripts of proteins, including N-methyl D-aspartate (NMDA) receptor 1 (Grin1) and a calcium ATPase (Atp2b1), that play important roles in neuronal excitation and calcium homeostasis.

Immunogeno

GST-tagged recombinant protein corresponding to mouse Fox1.

Applicazioni

Anti-Fox1 Antibody, clone 1D10, Cat. No. MABE985, is a highly specific mouse Monoclonal antibody, that targets RNA binding protein fox-1 homolog 1 and has been tested in Western Blotting, Immunocytochemistry, Immunohistochemistry and RNA-Binding Immunoprecipitation/iCLIP.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Fox1 in 10 µg of adult mouse frontal cortex tissue lysate.
Immunohistochemistry Analysis: A representative lot detected Fox1 in coronal sections from E18 mouse (Tang, Z.Z., et al. (2009). Mol Cell Biol. 29(17):4757-4765).
Immunocytochemistry Analysis: A representative lot detected Fox1 in post-mitotic neuronal cells differentiated from mouse embryonal carcinoma P19 cells (Lee, J.A., et al. (2009). Genes Dev. 23(19):2284-2293).
RNA-Binding Protein Immunoprecipitation/iCLIP Analysis: A representative lot was employed to immunoprecipitate Fox1-associated pre-mRNAs from UV-crosslinked murine cortex homogenates by individual-nucleotide resolution crosslinking immunoprecipitation (iCLIP) for characterization of Fox1 pre-mRNA-binding sites (Gehman, L.T., et al. (2011) Nat Genet. 43(7):706-711).
Western Blotting Analysis: A representative lot detected Fox1 in mouse cortical tissue homogenate (Tang, Z.Z., et al. (2009). Mol Cell Biol. 29(17):4757-4765).

Qualità

Evaluated by Western Blotting in adult mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fox1 in 10 µg of adult mouse brain tissue lysate.

Descrizione del bersaglio

~43 kDa observed

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable