MABC953

Anti-Prohibitin-2/PHB2 Antibody, clone 7F8E3

clone 7F8E3, from rat

• Sinonimo/i: Prohibitin-2, B-cell receptor-associated protein BAP37, D-prohibitin, Repressor of estrogen receptor activity, Prohibitin-2/PHB2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rat
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 7F8E3, monoclonal
• Reattività contro le specie: rat, monkey, human, mouse
• tecniche: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_001138303
• N° accesso UniProt: Q99623
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... PHB2(11331)

Descrizione

Descrizione generale

Prohibitin-2 (UniProt Q99623; also known as B-cell associated protein, B-cell receptor-associated protein BAP37, BCAP37, D-prohibitin, PHB2, Repressor of estrogen receptor activity) is encoded by the PHB2 (also known as BAP, REA) gene (Gene ID 11331) in human. Prohibitins are assembled into a ring-like PHB complex with 16-20 alternating Phb1 and Phb2 subunits in the inner mitochondrial membrane, where they function as chaperone for respiration chain proteins or as important structural scaffold for optimal mitochondrial morphology and function. Prohibitins are also reported to modulate transcriptions by interacting with various transcription factors, including nuclear receptors. Upregulated expression of prohibitins, septin 9, neurabin 1, and other cytoskeletal and functional proteins is found at the glutathione S-transferase placental-form-positive (GST-P+) hepatocellular carcinoma (HCC) foci during initiation and/or promotion stages of rat hepatocarcinogenesis, implicating an involvement of prohibitins in the neoplastic transformation of rat liver preneoplastic lesions.

Specificità

Expected to react with both spliced isoforms.

Immunogeno

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human Prohibitin-2/PHB2.

Applicazioni

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Prohibitin-2/PHB2 Antibody, clone 7F8E3 is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin) for the detection of Prohibitin-2/PHB2.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Prohibitin-2/PHB2 in 10 µg of COS-7 cell lysate.
Immunocytochemistry Analysis: 4 µg/mL from a representative lot detected Prohibitin-2/PHB2 in A431 and HUVEC cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Prohibitin-2/PHB2 in human breast and mouse liver tissue.
Immunohistochemistry Analysis: A representative lot detected upregulated Prohibitin-2 immunoreactivity at the glutathione S-transferase placental-form–positive (GST-P+) hepatocellular carcinoma (HCC) foci in frozen liver sections from rats subjected to hepatocarcinogenesis induction by N-nitrosodiethylamine (DEN) and Phenobarbital (PB) treatment (Kakehashi, A., et al. (2011). Toxicol Sci. 119(1):61-72).

Qualità

Evaluated by Western Blotting in rat C6 glioma cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Prohibitin-2/PHB2 in 10 µg of rat C6 glioma cell lysate.

Descrizione del bersaglio

~34 kDa observed

Stato fisico

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable